Dustri Online Services

Search Results

Books: 0	Journals: 1	Articles: 1

V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)

Effect of deuterium on the morpho-functional characteristics of normal and cancer cells in vitro

Ihor A. Zlatskiy, Alona V. Zlatska, Nadine V. Antipova, and Anton V. Syroeshkin

Price
42.00 $

Add to Cart

Price

42.00 $

Volume 35 (2018) p. 211 - 214

Abstract

Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (211-214)

Effect of deuterium on the morpho-functional characteristics of normal and cancer cells in vitro

Ihor A. Zlatskiy¹#², Alona V. Zlatska³#⁴, Nadine V. Antipova¹#⁵, and Anton V. Syroeshkin¹

¹Peoples Friendship University of Russia (RUDN University), Moscow, Russia, ²Dumanskii Institute of Colloid and Water Chemistry, National Academy of Science of Ukraine, ³State Institute of Genetic and Regenerative Medicine of NAMS of Ukraine, ⁴Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya, Kiev, Ukraine, and ⁵Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia
Objective: The aim of our study was to describe effects of different deuterium concentration on morphology and migratory activity of normal stem cells and cancer cell lines in vitro. Materials and methods: Water with different deuterium content was used for the culture media preparation: deuterium-depleted water (ddw, D/H = 1 ppm), deutereted (deuterium-rich) water (D/H = 99 abs. At. D%); water with natural deuterium content (MiliQ system) (D/H = 150 ppm) served as control. The cells were cultured in DMEM: F12 supplemented with 10% FBS, 2 mM L-glutamine, and 1 ng/mL FGF-2 in a multi-gas incubator at 5% CO₂ and 5% O₂. The morphology of adipose-derived mesenchymal stem cells (ADSCs) was observed after 24 and 72 hours cultivation in experimental media. After incubating for 0, 12, 24, and 48 hours, the gap width of scratch re-population was measured and recorded, and then compared with the initial gap size at 0 hours. Results: High deuterium concentration in culture medium leads to significant morphological changes in normal ADSCs that are associated with cellular stress. Moreover, the migratory activity of ADSCs was inhibited under the deutereted water. At the same time, ddw did not influence morphology or migration of ADSCs. Bothdeutereted water and ddw strongly inhibited migration of cancer cell lines A549 and HT29. Conclusion: Our findings demonstrated that deuterium could act as regulator of biological properties of normal and cancer cells in vitro.  However, the mechanisms that underlie the deuterium-mediated effect on different cellular types need to be further investigated.Correspondence to:
Ihor A. Zlatskiy, PhD
Peoples Friendship University of Russia (RUDN University)
6 Miklukho-Maklaya St, Moscow, 117198, Russia
Email: [email protected]

Shopping Overview

Shopping Overview
Type	Qtty	Discount	VAT	Price
Your basket is empty

View Cart

Search Results

Shopping Overview

Send us an E-Mail