Volume 35 (2018), No. 4/2018(4th quarter)
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Original
Vitamin D for cancer patients in routine health care – the patients’ perspective
Sabine Kirton, Uta Stutz, Oliver Micke, and Jutta Hübner
Price
42.00 $
Page No. 163
Abstract
Vitamin D for cancer patients in routine health care – the patients’ perspective
Sabine Kirton1, Uta Stutz2, Oliver Micke3, and Jutta Hübner4
1Women’s Cancer Support Association, Landesverband Mecklenburg-Vorpommern/Schleswig-Holstein e.V., Ostseebad Heringsdorf, 2Patient Information Center, University Hospital Greifswald, Greifswald, 3Franziskus Hospital, Department of Radiation Oncology, Bielefeld, and 4Medical Department of Hematology and Medical Oncology, University Hospital Jena, Jena, Germany
Background: In cancer care, risk of or manifest osteoporosis is an indication to optimize 25(OH) levels. Little is known about the supply of Vitamin D in routine cancer care in Germany. Our aim was to get first data on the medical care situation in Germany. Materials and methods: Together with the Women’s Cancer Support Association we developed a questionnaire and tested the pilot version with leading members of cancer self-help groups. The standardized questionnaire comprises data concerning bone density measurement, vitamin D (testing of blood level, prescription), and information and communication regarding vitamin D. Results: 47 patients took part in our pilot survey. 47% of the participants reported that the topic of vitamin D had been raised by a physician, and 36% had brought up the topic themselves. 57% rated communication as good or very good. While 68% reported having had bone density measurement, only 58% of those with low bone mineral density reported having had a test of the vitamin D level. 80% of the tested persons reported a low level; from those, 80% got a prescription. Most often 20,000 IU/week was prescribed (81.3% of those with a prescription). 35% reported not having had further tests. 15% of all participants reported taking vitamin D on their own initiative. The most important source of information, besides physicians, are self-help groups. Conclusion: Even in case of proven deficiency, a considerable rate of patients are not substituted. Most probably, undersupply will be more prominent in patients with no contact to self-help groups. More efforts should be made to inform physicians and patients of the importance of vitamin D.
Correspondence to:
Prof. Dr. Jutta Hübner
Medizinische Klinik II
Hämatologie und Internistische Onkologie
Universitätsklinikum Jena
Am Klinikum 1, 07747 Jena, Germany
Email: jutta.huebner@
med.uni-jena.de
Original
Effects of trans fatty acids intake on essential elements in liver and kidney of rats
Shuang Nie, Ping Liu, Tiantian Zhang, Yu Sun, and Na Ren
Price
42.00 $
Page No. 169
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (169-173)
Effects of trans fatty acids intake on essential elements in liver and kidney of rats
Shuang Nie, Ping Liu, Tiantian Zhang, Yu Sun, and Na Ren
Department of Hygiene Detection, College of Public Health, Shandong University, Jinan, Shandong, China
Over time, trans fatty acids (TFAs) have appeared more and more in people’s lives, and the harm of TFAs has been gradually discovered and confirmed. Excessive intake of trans fats can cause metabolic disturbance and increase the risk of coronary heart disease (CHD). In addition, TFAs are also closely related to diabetes, Alzheimer’s disease, and certain cancers, and have adverse effects on the health of pregnant women and the growth of infants. Trace elements, as bioactive substances in the organism, play an important role in normal physiological functions and maintaining the normal metabolism of organisms. We aim to investigate possible changes in some essential elements in liver and kidney of rats exposed to long-term intake of TFAs. Rats fed with different doses of TFAs were divided into different dosage groups during 12 weeks, namely low-dose group (50 mg/kg×BW), medium-dose group (100 mg/kg×BW) and high-dose group (150 mg/kg×BW). At the end of the exposure period, the organs were taken from the rats and carefully preserved. The levels of certain elements (Ca, Mg, Zn, Fe, Cu) were measured by using atomic absorption spectrophotometry (AAS). It was found that long-term intake of TFAs was associated with decreased hepatic Ca levels (p < 0.05) and with decreased renal Ca levels (p < 0.05). A statistically significant decrease was found in Cu levels in liver and kidney in the experimental groups compared with the control group (p < 0.05). Moreover, renal Fe levels were significantly decreased in low and medium groups (p < 0.05), while no significant differences were observed in Zn and Mg levels. In summary, the results of this study have shown that long-term intake of TFAs can influence mineral status, causing Cu and Ca deficiency in liver and kidney, but the mechanisms responsible for this phenomenon are unclear and require further investigation.
Correspondence to:
Prof. Ping Liu
Department of Hygiene Detection
College of Public Health, Shandong University
Jinan, Shandong, 250012, China
Email: [email protected]
Original
The role of selenoprotein P and selenium in the etiopathogenesis of gestational diabetes mellitus: Association with selenoprotein P1 gene (rs3877899) polymorphism
F. Behice Serinkan Cinemre1, Hakan Cinemre2, Ayse Yücel3, Sevgin Degirmencioglu4, Abdullah Tüten5, M. Aytaç Yüksel5, Nevin Yılmaz5, Tevfik Gulyasar6, Mustafa Yıldız6, Nurten Bahtiyar7, Ali Rıza Kızıler8, and Birsen Aydemir3
Price
42.00 $
Page No. 174
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (174-182)
The role of selenoprotein P and selenium in the etiopathogenesis of gestational diabetes mellitus: Association with selenoprotein P1 gene (rs3877899) polymorphism
F. Behice Serinkan Cinemre1, Hakan Cinemre2, Ayse Yücel3, Sevgin Degirmencioglu4, Abdullah Tüten5, M. Aytaç Yüksel5, Nevin Yılmaz5, Tevfik Gulyasar6, Mustafa Yıldız6, Nurten Bahtiyar7, Ali Rıza Kızıler8, and Birsen Aydemir3
1Department of Biochemistry, 2Department of Internal Medicine, 3Department of Biophysics, Medical Faculty, Sakarya University, Sakarya, 4Vocational School of Health Services, Istanbul Bilim University, 5Department of Obstetrics and Gynecology, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, 6Department of Biophysics, Medical Faculty, Trakya University, Edirne, 7Department of Biophysics, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul, and 8Department of Biophysics, Medical Faculty, Namık Kemal University, Tekirdag, Turkey
Objective: The aim of this study was to investigate the role of selenoprotein P (SeP) and selenium in the etiopathogenesis of gestational diabetes mellitus (GDM) and their association with a common selenoprotein P1 (rs3877899) single nucleotide polymorphism (SNP) in pregnant women with GDM. Materials and methods: Eighty-six pregnant women with GDM and 90 healthy pregnant women from the same geographic region were included in the study. Fasting glucose, insulin HOMA-IR, and HbA1c were compared. Serum selenium levels were measured by graphite-furnace atomic absorption spectrophotometer. Plasma SeP levels were determined by ELISA. Allele-specific polymerase chain reaction (ASPCR) analysis was used to identify polymorphisms of the selenoprotein P1 gene (SEPP1) (rs3877899). Results: The biochemical parameters of GDM such as fasting glucose, insulin, HOMA-IR, and HbA1c were higher in pregnant women with GDM compared to healthy pregnant women. Maternal selenium levels (µg/L) were 77.99 ± 7.21 and 76.04 ± 7.77 in GDM and healthy pregnant women, respectively (p > 0.05). However, SeP levels (ng/mL) were found to be significantly lower in GDM (35.29 ± 3.00) compared to control subjects (46.98 ± 4.59) (p < 0.01). Although there was no significant difference in the distribution of the SEPP1 genotypes and alleles between two groups, SeP levels were higher in the GG genotype of the gene compared to their respective control (p < 0.001). Conclusion: Although frequency of SEPP1 polymorphism and selenium levels did not differ significantly between diabetic and healthy pregnant women, SeP levels increased in pregnant women with GDM suggesting SeP plays a role in the etiopathogenesis of GDM. Moreover, the GG genotype of SEPP1 gene polymorphism may be involved in the development of GDM with a different mechanism. It should be clarified with further studies in larger populations.Correspondence to:
Fatma Behice Serinkan Cinemre, MD
Associate Professor of Biochemistry
Sakarya University School of Medicine
Department of Biochemistry
54290 Sakarya, Turkey
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Editorial
Prof. Margarita G. Skalnaya
Page No. 183
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (183)
Editorial
Prof. Margarita G. Skalnaya
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Intranasal exposure to manganese induces activation of calpains in rat striatum
Irina Oblamskaya, Nina Pestereva, Zamira Muruzheva, Maria Tikhomirova, and Marina Karpenko
Price
42.00 $
Page No. 184
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (184-186)
Intranasal exposure to manganese induces activation of calpains in rat striatum
Irina Oblamskaya1, Nina Pestereva1, Zamira Muruzheva1, Maria Tikhomirova2, and Marina Karpenko1,3
1Department of Physiology (Pavlov’s), Institute of Experimental Medicine, St. Petersburg, Russia, 2Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland, and 3Department of Biophysics, Institute of Physics, Nanotechnology and Telecommunications, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia
Chronic manganese (Mn) exposure causes a variety of psychiatric and motor disturbances, termed manganism. Here, we provide an evidence to support the involvement of the calcium-dependent proteases, calpains, in the pathogenesis of manganism in a rat model. 20 male rats were divided randomly into two groups: the control group and the Mn exposure group. The rats in the Mn exposure group received intranasal injection of 1 mg/rat MnCl2×4H2O once a day for 90 days. The control group rats received intranasal injection of physiological saline the same way. The levels of Mn in the striatum (Str) were assessed using atomic absorption spectroscopy. Mn levels were found to be significantly higher in the experimental group of rats than in the control group. Further, the involvement of calpain-1 and calpain-2 was monitored in the Str. The activity of calpain-1/2 was determined by casein zymography. It was revealed, that Mn induced an increase of the activity of calpain-2 in the Str, where the activity of calpain-1 was also increased. We have also shown that Mn directly activates calpain-1 like calcium but not calpain-2. We propose that calpains can be potential therapeutic targets for manganism.Correspondence to:
Irina Oblamskaya, PhD student
Department of Physiology (Pavlov’s)
Institute of Experimental Medicine
197376, Akademika Pavlova street, 12, St. Petersburg, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Regional assessment of the impact of mercury on the health of children
Natalya V. Stepanova and Suryana F. Fomina
Price
42.00 $
Page No. 187
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (187-189)
Regional assessment of the impact of mercury on the health of children
Natalya V. Stepanova and Suryana F. Fomina
Kazan Federal University (KFU), Institute of Fundamental Medicine and Biology, Kazan, Russia
The total level of mercury (Hg) in human hair is a major biomarker characterizing exposure to methylmercury (MeHg), which is mainly due to fish intake. Analysis of Hg intake with the diet and its content in the hair of children aged 3 – 7 years old from the city of Kazan was carried out. The study of child nutrition was carried out in two directions: individual and family nutrition (questionnaire method) and assessment of public catering (time-weight method). The Hg content was determined by means of inductively-coupled (argon) plasma mass-spectrometry (ICP-MS) on the ELAN-9000 instrument (PerkinElmer, USA). The indices of geometric mean level (GM) and the 95th percentile in all children were significantly below the limit values recommended by US-EPA (1.0 µg/g) and JECFA/WHO (1.9 µg/g of hair) for the most vulnerable population group. Our study showed a safety Hg exposure due to fish intake for children from Kazan.Correspondence to:
Prof. Natalya Vladimirovna Stepanova, MD
Kazan Federal University (KFU)
Institute of Fundamental Medicine and Biology
420008, Kazan, K. Marx str., 76, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Element status of people living in the Middle East territory
Bassam H. Kabki, Motlaq Al-Mohalep, and Anatoly A. Kirichuk
Price
42.00 $
Page No. 190
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (190-192)
Element status of people living in the Middle East territory
Bassam H. Kabki1, Motlaq Al-Mohalep2, and Anatoly A. Kirichuk1
1Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia, and 2Prevention and Treatment Laboratory, Hafer Al-Batin, Kingdom of Saudi Arabia
Element status of 581 subjects (345 women and 86 men 25 – 50 years old; 64 girls and 86 boys 3 – 15 years old) living in the Middle East was estimated using inductively-coupled plasma mass spectrometry (24 chemical elements). The obtained data were compared to available data on the content of chemical elements in human hair reported in literature from other regions of the world. The results showed that Middle East inhabitants are characterized by relatively low levels of cadmium, lead, mercury, arsenic (all groups), silicon, chromium, manganese (adults), aluminum, nickel, cobalt (adult women); high levels of iron (all groups except adult women), sodium, potassium (all groups), and an extreme hair content of boron (all groups, up to 5.02 µg/g median value). The above-mentioned features may occur due to geochemical characteristics of the territory, though contribution of other factors, such as local peculiarities of industry, agriculture, nutrition, and lifestyle, may also be significant.Correspondence to:
Dr. Bassam Kabki
Peoples’ Friendship University of Russia (RUDN University)
Miklukho-Maklaya str., 8, 117198, Moscow, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Alteration of mineral element status of rodents under combined group B vitamin deficiency
Sergey A. Apryatin, Antonina A. Shumakova, Oksana A. Vrzhesinskaya, Svetlana N. Leonenko, Vera M. Kodentsova, and Ivan V. Gmoshinski
Price
42.00 $
Page No. 193
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (193-195)
Alteration of mineral element status of rodents under combined group B vitamin deficiency
Sergey A. Apryatin, Antonina A. Shumakova, Oksana A. Vrzhesinskaya, Svetlana N. Leonenko, Vera M. Kodentsova, and Ivan V. Gmoshinski
Federal Research Center for Nutrition and Biotechnology, Moscow, Russia
Alterations in mineral element status have been assessed under the combined nutritional deficiency of B1, B2, and B6 vitamins in rat and murine in vivo models. Female Wistar (W) and hybrids of the 1st generation of Dark Agouti and Wistar (DA×W) strains, as well as female BALB/c and DBCB tetrahybrid mice were used. Animals were fed with a balanced diet (control groups) according to AIN93M or lacking vitamins B1, B2, B6 (experimental groups) for 5 weeks. The level of essential and toxic mineral elements has been estimated in kidneys by ICP-MS. DA×W rats compared to W and DBCB mice compared to BALB/c were more sensitive to the development of B-vitamin deficiency as assessed by the B-vitamin sufficiency markers. The content of Mg, Сu, Zn, and Cr was decreased, while Cs level was increased in the kidneys of the rats of the experimental groups. In mice, group B vitamin deficiency resulted in reduced Mg, Cu, Zn, Cr, Cd, and Pb content, and excessive Cs accumulation. Some of these biomarkers are supposed to be used in pre-clinical evaluation of the effectiveness of new vitamin dietary supplements, as well as studies of various vitamin interactions.Correspondence to:
Sergey A. Apryatin, PhD
Federal Research Centre for Nutrition and Biotechnology
Ustinskiy pr., 2/14, 109240, Moscow, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Examination of macro- and trace element content in hair of workers of a mining and processing enterprise in Mongolia
Odontsetseg Brown, Andrei R. Grabeklis, and Inna Y. Tarmaeva
Price
42.00 $
Page No. 196
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (196-199)
Examination of macro- and trace element content in hair of workers of a mining and processing enterprise in Mongolia
Odontsetseg Brown1, Andrei R. Grabeklis2#3, and Inna Y. Tarmaeva4
1Foundation for Health Policy Promotion, Ulaanbaatar, Mongolia, 2P.G. Demidov Yaroslavl State University, Yaroslavl, 3Peoples’ Friendship University of Russia (RUDN University), Moscow, and 4Irkutsk State Medical University, Irkutsk, Russia
Element status of 40 male workers (43.9 ± 8.9 years old) of the “Erdenet” copper-molybdenum ore mining and processing enterprise (Mongolia) was estimated using hair inductively-coupled plasma mass-spectrometry analysis and comparison with outer control group. Differences between workers of the production (PS) and non-production (NPS) spheres were also studied. It was found that Mongolian workers do not differ from the control group by levels of macroelements though have significantly lower hair content of Cr (2.1-fold), I (4.4-fold), Se (1.2-fold), Cd (1.6-fold), Hg (4.2-fold), Si (1.5-fold), V (3.3-fold), and much higher hair content of Mo (9.5-fold). PS workers were found to have higher hair Mo (1.6-fold, p < 0.001) than NPS. Significantly higher hair content of essential Co (3.3-fold), Cr (1.9-fold), Fe (2-fold), I (3-fold), Se (1.2-fold), and Ca (1.7-fold) was also observed in PS workers. The data indicate distinct effect of occupational exposure to Mo. Lowered levels of essential elements, especially pronounced in the NPS subgroup, probably reflect peculiarities of Mongolian diets lacking vegetables and sea food.Correspondence to:
Andrei R. Grabeklis, PhD
Peoples’ Friendship University of Russia (RUDN University)
Moscow, 117198, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Ecological-geochemical characteristics of lead levels in the environment and human biosubstrates of residents of the Orenburg region
Elena V. Salnikova, Tatyana I. Burtseva, Aleksei N. Sizentsov, Tatyana F. Tarasova, Alina I. Bailetova, Olga V. Kvan, Galina V. Karpova, and Sergei A. Pezhkov
Price
42.00 $
Page No. 200
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (200-202)
Ecological-geochemical characteristics of lead levels in the environment and human biosubstrates of residents of the Orenburg region
Elena V. Salnikova1, Tatyana I. Burtseva1, Aleksei N. Sizentsov1, Tatyana F. Tarasova1, Alina I. Bailetova1, Olga V. Kvan2, Galina V. Karpova1, and Sergei A. Pezhkov1
1Orenburg State University and 2Federal Research Centre of Biological Systems and Agro-technologies of the Russian Academy of Sciences, Orenburg, Russia
Data on the content of lead in water, the soil, wheat, and hair of inhabitants of the western, central, and eastern areas of the Orenburg region are presented in this article. 525 samples of water, soil, and wheat and 420 samples of human hair were studied using atomic absorption and mass spectrometry with inductively-coupled plasma, respectively. It is estimated that in underground waters, the soil, and wheat of the eastern area the content of lead is the highest in comparison with the western and central areas of the region. In hair of inhabitants of the eastern area, the level of lead exceeded the respective values in the western and central areas by a factor of 2.2 (p < 0.001) and 1.5 (p < 0.001), respectively.Correspondence to:
Elena V. Salnikova, PhD, Assoc. Prof.
Orenburg State University
Pobedy Ave. 13, Orenburg, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Estimation of toxic properties of nanocomposites containing nanoparticles of bismuth, gadolinium, and silver
Viktor Stepanovich Rukavishnikov, Mikhail Aleksandrovich Novikov, Evgeny Alekseevich Titov, Larisa Mikhailovna Sosedova, Vera Aleksandrovna Vokina, and Natal’ya Leonidova Yakimova
Price
42.00 $
Page No. 203
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (203-206)
Estimation of toxic properties of nanocomposites containing nanoparticles of bismuth, gadolinium, and silver
Viktor Stepanovich Rukavishnikov1#2, Mikhail Aleksandrovich Novikov1#2, Evgeny Alekseevich Titov1#2, Larisa Mikhailovna Sosedova1#2, Vera Aleksandrovna Vokina1, and Natal’ya Leonidova Yakimova1
1East-Siberian Institute of Medical and Ecological Research, Angarsk, and 2Irkutsk Scientific Center, Siberian Branch of Russian Academy of Science, Irkutsk, Russia
The aim of the study was to study the parameters of acute toxicity, gender effects, and activity of apoptosis modulator proteins bcl-2, caspase-3, and hsp70 in albino rat brain neurons in response to administration of silver, bismuth, gadolinium nanoparticles-containing nanocomposites. Parameters of acute toxicity of nanobiocomposites were determined by intragastric administration. Subacute toxicity was studied by intragastric administration of nanocomposites for 9 days at a rate of 500 µg/kg body weight. Immunohistochemical method was used for assessment of modulators of apoptosis of bcl-2 and caspase-3 in albino rat brain neurons and the activity of heat shock proteins. All nanobiocomposites belong to a low-hazard class of substances on the parameters of acute toxicity. At the same time, in a subacute experiment, despite the same matrix, the response of the organism was specific depending on the metal nanoparticles encapsulated in the matrix. Administration of silver nanocomposites caused proapoptotic effect in brain cells. Bismuth and gadolinium nanocomposites did not stimulate of programmed cell death in brain.Correspondence to:
Larisa Mikhailovna Sosedova
FSBSI “Eastern-Siberian Institution of Medical and Ecological Research”
665827, Russia, Irkutsk region,
Angarsk, district 12a, 3, PO Box 1170, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Comparative analysis of elemental status in benign and malignant brain tumors
Kirill V. Kuzmichev, Elena I. Erlykina, Larisa M. Obukhova, Igor A. Medyanik, Konstantin S. Yashin, Tatiana I. Storozheva, Ilya I. Evdokimov, and Vladimir G. Pimenov
Price
42.00 $
Page No. 207
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (207-210)
Comparative analysis of elemental status in benign and malignant brain tumors
Kirill V. Kuzmichev1, Elena I. Erlykina1, Larisa M. Obukhova1, Igor A. Medyanik1, Konstantin S. Yashin1, Tatiana I. Storozheva2, Ilya I. Evdokimov2, and Vladimir G. Pimenov2
1Privolzhsky Research Medical University and 2G.G. Devyatykh Institute of Chemistry of High-Purity Substances of the Russian Academy of Sciences, Nizhny Novgorod, Russ
The parameters of mineral metabolism in blood plasma and tumor tissue were analyzed in 12 patients with malignant and 7 patients with benign brain tumors before treatment. As a control, the brain tissue was used from the corpses of 7 people who died as a result of trauma and blood from 10 practically healthy people. The level of macroelements and trace elements was evaluated by atomic-emission spectrometry. A statistically significant increase in the concentration of Ca, Na and a decrease in Mg, K, P in tumor tissue of the brain was revealed in comparison with the brain tissue of the control group and patients with benign tumors. The level of trace elements in the tumor tissue also changed: the content of Fe, Zn, Cu, Se, Li, and Sr increased by a factor of 3.5, 2.9, 4.3, 2.3, 2.4, and 5, respectively. When analyzing the element levels of blood plasma, there was no significant change in the Na level; the content of K, Ca, Mg, and P increased in comparison with the results of the control group by a factor of 2.3, 1.3, 16, and 3.14, respectively. In the blood plasma, the content of Fe, Zn, Li, and Sr also increased by a factor of 2, 2.1, 2.7, and 4, respectively, and Cu and Se, on the contrary, decreased by a factor of 1.7 and 1.8.Correspondence to:
Kirill V. Kuzmichev, BSc
Privolzhsky Research Medical University
Minin Sq., 10/1, 603950, Nizhny Novgorod, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Effect of deuterium on the morpho-functional characteristics of normal and cancer cells in vitro
Ihor A. Zlatskiy, Alona V. Zlatska, Nadine V. Antipova, and Anton V. Syroeshkin
Price
42.00 $
Page No. 211
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (211-214)
Effect of deuterium on the morpho-functional characteristics of normal and cancer cells in vitro
Ihor A. Zlatskiy1#2, Alona V. Zlatska3#4, Nadine V. Antipova1#5, and Anton V. Syroeshkin1
1Peoples Friendship University of Russia (RUDN University), Moscow, Russia, 2Dumanskii Institute of Colloid and Water Chemistry, National Academy of Science of Ukraine, 3State Institute of Genetic and Regenerative Medicine of NAMS of Ukraine, 4Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya, Kiev, Ukraine, and 5Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia
Objective: The aim of our study was to describe effects of different deuterium concentration on morphology and migratory activity of normal stem cells and cancer cell lines in vitro. Materials and methods: Water with different deuterium content was used for the culture media preparation: deuterium-depleted water (ddw, D/H = 1 ppm), deutereted (deuterium-rich) water (D/H = 99 abs. At. D%); water with natural deuterium content (MiliQ system) (D/H = 150 ppm) served as control. The cells were cultured in DMEM: F12 supplemented with 10% FBS, 2 mM L-glutamine, and 1 ng/mL FGF-2 in a multi-gas incubator at 5% CO2 and 5% O2. The morphology of adipose-derived mesenchymal stem cells (ADSCs) was observed after 24 and 72 hours cultivation in experimental media. After incubating for 0, 12, 24, and 48 hours, the gap width of scratch re-population was measured and recorded, and then compared with the initial gap size at 0 hours. Results: High deuterium concentration in culture medium leads to significant morphological changes in normal ADSCs that are associated with cellular stress. Moreover, the migratory activity of ADSCs was inhibited under the deutereted water. At the same time, ddw did not influence morphology or migration of ADSCs. Bothdeutereted water and ddw strongly inhibited migration of cancer cell lines A549 and HT29. Conclusion: Our findings demonstrated that deuterium could act as regulator of biological properties of normal and cancer cells in vitro.
However, the mechanisms that underlie the deuterium-mediated effect on different cellular types need to be further investigated.Correspondence to:
Ihor A. Zlatskiy, PhD
Peoples Friendship University of Russia (RUDN University)
6 Miklukho-Maklaya St, Moscow, 117198, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Effect of zinc-containing nanoparticles on cytomorphological and biochemical parameters in rats
Elena A. Sizova, Sergey A. Miroshnikov, and Elena V. Yausheva
Price
42.00 $
Page No. 215
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (215-217)
Effect of zinc-containing nanoparticles on cytomorphological and biochemical parameters in rats
Elena A. Sizova1#2, Sergey A. Miroshnikov1, and Elena V. Yausheva1
1Federal Research Center of Biological Systems and Agro-technologies of the Russian Academy of Sciences and 2Orenburg State University, Orenburg, Russia
The aim of this research was to study the response of the rat organism (the level of serum aminotransferases, proinflammatory cytokines, caspase-3 expression) to the administration of zinc (ZnNPs) and its oxide (ZnONPs) nanoparticles. The results indicate the hepatotoxic effect of the studied nanoparticles (NPs). Increased activity of serum aspartate aminotransferases (AST) and alanine aminotransferases (ALT), markers of inflammation, activation of the proapoptic protein caspase-3, capillary-trophic insufficiency, and the induction of proinflammatory cytokines in animals administered with high doses of ZnONPs have been determined. In conclusion, ZnONPs (with comparable administration doses) are more toxic than ZnNPs.Correspondence to:
Elena A. Sizova, PhD, DSc.
Biology Federal Research Centre of Biological Systems and
Agro-technologies of the Russian Academy of Sciences
29, ul. 9 Yanvarya, Orenburg, 460000 Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
The association between chronic sinusitis and hair ultra-trace element levels in hair of children
Svetlana I. Alekseenko, and Anatoly V. Skalny
Price
42.00 $
Page No. 218
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (218-220)
The association between chronic sinusitis and hair ultra-trace element levels in hair of children
Svetlana I. Alekseenko1#2, and Anatoly V. Skalny3#4#5
1Raukhfus Children’s City Hospital No. 19, 2Mechnikov North-West State Medical University, St. Petersburg, 3Yaroslavl State University, Yaroslavl, 4Peoples’ Friendship University of Russia (RUDN University), and 5All-Russian Research Institute of Medicinal and Aromatic Plants (VILAR), Moscow, Russia
The objective of the present study was to assess the association between hair levels of ultra-trace elements and chronic sinusitis in children. Hair samples of 66 children with chronic sinusitis (J32.0 – ICD-10) (mean age – 11.2 ± 4.5 years) and 66 gender-matched controls (mean age – 11.3 ± 4.6 years) were analyzed for Ag, Au, Ba, Bi, Ga, Ge, La, Mo, Pt, Rb, Sb, Tl, W, and Zr content using inductively-coupled plasma mass spectrometry at NexION 300D (PerkinElmer Inc., Shelton, CT, USA) after microwave digestion of hair samples. The obtained data demonstrate that hair Ga, Tl, and Zr were significantly increased by 20% (p = 0.004), 33% (p = 0.006), and 36% (p = 0.047), respectively, as compared to the control values. In turn, hair Pt levels were 2-fold higher in the group of patients when compared to the healthy controls (p = 0.003). A nearly significant (p = 0.066) 50% increase in hair Sb was observed. Correlation analysis demonstrated that in a group of healthy children, hair Au (–0.2657; p = 0.031), Sb (–0,3871; p = 0.001), W (–0,3046; p = 0.013), and Zr (–0.2766; p = 0.025) levels were significantly inversely correlated with age of the examinees, whereas no such associations were revealed in the patient group. We hypothesize that altered ciliary apparatus in chronic sinusitis patients may result in reduced removal of inhaled metal particles and their subsequent toxicity.Correspondence to:
Anatoly V. Skalny, MD, PhD, Dsc
Peoples’ Friendship University of Russia (RUDN University)
Moscow, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Neutron flow effect on metabolic characteristics of Artemia salina cysts
Alla V. Marukhlenko, Maria A. Morozova, and Tatiana V. Pleteneva
Price
42.00 $
Page No. 221
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (221-224)
Neutron flow effect on metabolic characteristics of Artemia salina cysts
Alla V. Marukhlenko, Maria A. Morozova, and Tatiana V. Pleteneva
RUDN University, Moscow, Russia
The research object of this work is the effect of the background thermal neutron flux on metabolic rates of living systems on the example of the microelement composition of diapausing Artemia salina cysts. We have studied the metabolic characteristics of alive and dead A. salina cysts before and after artificial neutron flux exposure. The irradiation unit consisted of a neutron source INK1-06 (252Cf, activity – 1.42×104 n/s ± 8%) with a lay of polyethylene for neutron thermalization. To assess the trace element composition of the cysts, a method of atomic absorption spectroscopy with electrothermal atomization and Zeeman background correction was used. It is demonstrated, that the concentrations of some metals in samples of alive, dead, and alive cysts irradiated with an artificial flow of thermal neutrons are not equal. The content of Mn in alive A. salina samples have increased more than 10-fold after their interaction with neutron flux, while the amount of As decreased by a factor of 2 after exposure. Levels of other elements (Al, Cr, Ni, Cu, Cd, Pb) did not show any significant difference.Correspondence to:
Alla V. Marukhlenko
RUDN University
Miklukho-Maklay str. 6, 117198, Moscow, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Relationship between anthropometric data, element status, and nutrition in Tajik schoolchildren
Anatoly A. Kirichuk, Yulia N. Lobanova, Andrey A. Skalny, Jamshed S. Dodkhoyev, and Anatoly V. Skalny
Price
42.00 $
Page No. 225
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (225-227)
Relationship between anthropometric data, element status, and nutrition in Tajik schoolchildren
Anatoly A. Kirichuk1, Yulia N. Lobanova1, Andrey A. Skalny1, Jamshed S. Dodkhoyev2, and Anatoly V. Skalny1#3#4
1RUDN University, Moscow, Russia, 2Avicenna Tajik State Medical University, Dushanbe, Tajikistan, 3P.G. Demidov Yaroslavl State University, Yaroslavl, and 4All-Russian research Institute of medicinal and aromatic plants (VILAR), Moscow, Russia
The aim of the study was to investigate the association between anthropometric data, element and nutritional status of Tajik schoolchildren within the national school feeding program. A total of 283 schoolchildren aged 7 – 10 years old (141 boys and 142 girls) attending grades 1 – 4 in schools on the territory of the Republic of Tajikistan (Ayni district) were examined. The study included physical examination, interview, and the collection of anthropometric data. Analysis of hair trace element and mineral (Al, As, B, Be, Ca, Cd, Co, Cr, Cu, Fe, Hg, I, K, Li, Mg, Mn, Na, Ni, P, Pb, Se, Si, Sn, V, Zn) content was performed using inductively-coupled plasma mass spectrometry. The obtained data showed the significant positive correlation between the anthropometric parameters, nutritional and element status of the examined schoolchildren. The higher risk of Cu deficiency is a particular problem of child nutrition in the studied region. Micronutrient-fortified nutrition (WFP program) significantly improved anthropometric data and element status of Tajik schoolchildren within the WFP program.Correspondence to:
Anatoly V. Skalny, MD, Prof.
RUDN University
6 Miklukho-Maklaya str., 117198, Moscow, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Influence of fast-food products on liver element composition and metabolic parameters of laboratory animals
Svetlana V. Notova, Elena V. Kiyaeva, Inara E. Laryushina, and Ainagul K. Duskaeva
Price
42.00 $
Page No. 228
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (228-231)
Influence of fast-food products on liver element composition and metabolic parameters of laboratory animals
Svetlana V. Notova1#2, Elena V. Kiyaeva1, Inara E. Laryushina1, and Ainagul K. Duskaeva1
1Orenburg State University and 2Federal Research Center for Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia
Objective: The aim of the work was to study the influence of fast-food and instant-food products on metabolic indicators and element status of laboratory animals. Materials and methods: The study was conducted on male Wistar rats. The experimental group consumed a semi-synthetic diet consisting of mixture of basic diet (50%), fast-food and instant food (50%). Total protein, ALT, AST, total bilirubin and triiodothyronine levels were assessed. The element composition of the liver was determined by atomic-emission and mass-spectrometry with inductively-coupled argon plasma. The activities of Cu, Zn-superoxide dismutase, and glutathione peroxidase were measured in erythrocyte lysate by spectrophotometric method. Results: A significant decrease in the content of a number of essential elements: Mn (3.6-fold), Se (1.21-fold), Si (3.6-fold), and Zn (1.25-fold) was observed. Al and Cd contents in the experimental group were significantly increased by a factor of 3.9 and 2, respectively. Consumption of fast-food products was accompanied by significantly lower values of total protein and greater activity of AST and ALT in serum. Activity of glutathione peroxidase (140.5 (121.0 – 151.0) U/mL) was significantly decreased (р = 0.000) in the experimental group in comparison to the control group (240.0 (229.0 – 251.0) U/mL).Correspondence to:
Prof. Dr. Svetlana V. Notova
Orenburg State University
Pobedy Ave. 13, Orenburg, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Gender-specific differences in hair rare trace element content in children with Down’s syndrome
Anatoly V. Skalny, Andrey R. Grabeklis, Anastasia A. Skalnaya, Anna L. Mazaletskaya, Margarita G. Skalnaya, and Alexey A. Tinkov
Price
42.00 $
Page No. 232
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (232-234)
Gender-specific differences in hair rare trace element content in children with Down’s syndrome
Anatoly V. Skalny1#2#3#4, Andrey R. Grabeklis1#2, Anastasia A. Skalnaya5, Anna L. Mazaletskaya1, Margarita G. Skalnaya1#2, and Alexey A. Tinkov1#2
1Yaroslavl State University, Yaroslavl, 2Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia, 3Trace Element Institute for UNESCO, Lyon, France, 4Orenburg State University, Orenburg, and 5Lomonosov Moscow State University, Moscow, Russia
The primary objective of the present study was to assess the levels of hair ultra-trace elements in patients with Down’s syndrome and the potential gender effects. A total of 50 children with Down’s syndrome (70% boys, 30% girls) aged 0 – 5 years and 50 age- and gender-matched neurotypical controls were enrolled in the present study. Proximal parts of hair strands (0.5 – 1 cm) were collected in a quantity of 0.05 – 0.1 g. Prior to analysis the samples were washed with acetone and distilled water and subjected to microwave digestion. The obtained data demonstrate that children with Down’s syndrome are characterized by a significant more than 2-fold increase in hair Ag levels as compared to the healthy controls. In turn, hair Pt levels in Down’s syndrome patients was 43% lower in comparison to the control values. Notably, hair Rb in patients with trisomy 21 had 63% higher hair Rb levels as compared to the respective control levels, although the difference was nearly significant. Further analysis revealed that the observed difference is gender-specific: hair Ag levels were significantly increased only in female examinees, whereas significantly lower hair Pt levels were observed only in boys with Down’s syndrome. Although the clinical significance of the revealed associations is unclear, one can propose that altered rare element levels may be associated with impaired metal-binding protein levels in Down’s syndrome.Correspondence to:
Prof. Anatoly V. Skalny, MD, PhD
Peoples’ Friendship University of Russia (RUDN University)
Miklukho-Maklaya Str., 6, Moscow, 105064, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Features of element status in children with congenital lung malformations
Galina Evseeva, Stefaniya Suprun, Ol’ga Lebed’ko, Sabina Pichugina, Evgenij Iakovlev, Mariya Kuznetsova, and Elena Knizhnikova
Price
42.00 $
Page No. 235
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (235-238)
Features of element status in children with congenital lung malformations
Galina Evseeva, Stefaniya Suprun, Ol’ga Lebed’ko, Sabina Pichugina, Evgenij Iakovlev, Mariya Kuznetsova, and Elena Knizhnikova
Far Eastern Scientific Center of Physiology and Pathology of Respiration – Khabarovsk Branch of Research Institute of Maternity and Childhood Protection, Khabarovsk, Russia
Objective: The aim of this work was to study the levels of trace elements in serum and blood cells in children with congenital lung malformations living in the Amur region, and to assess the significance of their imbalance for health. Materials and methods: 75 children with congenital lung malformations were examined during aggravation and remission of disease (average age 9.2 ± 3.2 years, average disease duration 6.4 ± 2.3 years). The Zn, Fe, Pb, Mo, Ni, Li, Mn, Cu, Co, Se, Al, Cd contents in blood cells and serum were determined by atomic absorption spectroscopy. The content of iodides in whole blood was determined by ionometry method. The control group consisted of 189 healthy children matched by sex and age. Results: Significant changes in the concentration of elements were characterized by the reduction of iodides (p < 0.001) during aggravation of the disease, Cu in blood cells both during aggravation (p < 0.001) and remission (p < 0.05), Mn in blood cells during aggravation (p < 0.05), Se in blood cells in remission (p < 0.001), Zn in serum (p < 0.001), as well as elevation of Pb levels (p < 0.001) both during aggravation and in remission. In the case of low iodides, Cu and Se concentration, the risk of disease aggravation was 5.4, 3, and 5-fold higher, respectively. Conclusion: The results of the study show that decreased blood essential trace elements levels may be indicative of the depletion of their reserves in case of chronic inflammation in the lungs, which can reduce the effectiveness of defensive mechanisms in the body and lead to disease aggravation. Thus, in the course of comprehensive treatment of COPD in children it is necessary to fill the deficit of iodine, Cu, Zn, and Se.Correspondence to:
Evseeva Galina, MD, PhD, DSc
Far Eastern Scientific Center of Physiology and Pathology of Respiration –
Khabarovsk Branch of Research Institute of Maternity and Childhood Protection
Khabarovsk, 680022, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Hair heavy metals and functional reserves of the organism in children of different ages in Moscow megapolis
Alexander E. Severin, Vladimir I. Torshin, Elena A. Severina, and Ivan V. Radysh
Price
42.00 $
Page No. 239
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (239-241)
Hair heavy metals and functional reserves of the organism in children of different ages in Moscow megapolis
Alexander E. Severin1, Vladimir I. Torshin1, Elena A. Severina1, and Ivan V. Radysh2
1Department Normal Physiology and 2Department Management of Nursing Activities, RUDN University, Moscow, Russia
The purpose of the research was to determine the association between hair heavy metal levels in children of different ages with the level of functional reserves of the organism in Moscow. Two groups (n = 78) of healthy boys living in Moscow aged 4 – 6 and 7 – 11 years were examined. Hair levels of Cr, Mn, Fe, Co, Ni, Cu, Zn, Cd, and Pb were assessed using atomic absorption spectrometry. Functional reserves of the body were estimated using computer spirometry and analysis of heart rate variability. Hair heavy metal content in children aged 7 – 11 years was significantly higher than that in the younger children for the majority of the elements studied, especially Cr, Fe, Zn, and Cd, whereas hair Ni content was higher in the younger children. Hair iron content significantly correlated with age (r = 0.38, p < 0.05). In contrast, the parameters of respiratory and cardiovascular function were increased in the older children. Therefore, the observed levels of inorganic pollutants in the hair of children did not significantly affect the growth and development of the organism and the formation of functional reserves. However, adverse health effects may occur at higher rate of urban environment pollution, being typical for certain areas of Moscow.Correspondence to:
Ivan V. Radysh, MD, PhD
Department Management of nursing activities
RUDN University
Miklukho–Maklaya St. 6, Moscow 117198, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Influence of various chromium compounds on physiological, morpho-biochemical parameters, and digestive enzymes activity in Wistar rats
Svyatoslav Lebedev, Irina Gavrish, Elena Rusakova, Olga Kvan, and Ilmira Gubaidullina
Price
42.00 $
Page No. 242
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (242-245)
Influence of various chromium compounds on physiological, morpho-biochemical parameters, and digestive enzymes activity in Wistar rats
Svyatoslav Lebedev1#2, Irina Gavrish1#2, Elena Rusakova1#2, Olga Kvan1#2, and Ilmira Gubaidullina1
1Federal Scientific Center “Biological Systems and Agrotechnologies RAS” and 2Orenburg State University, Orenburg, Russia
The aim of the study was to assess the complex response of rats to supplementation of various forms (Cr2O3 nanoparticle (NP), picolinate (CrPic) and CrCl3) and doses (300 and 500 µg/kg) of chromium in the diet of rats. The maximal daily weight gain was observed in rats fed with NP (p ≤ 0.05). The number of erythrocytes and hemoglobin in groups receiving chromium at a dose of 300 µg/kg increased in association with moderate leukocytosis (p ≤ 0.05). A a dose of 500 µg/kg of NP resulted in decreased ALT activity. The intake of CrCl3 (500 µg/kg) contributed to a decrease in the activity of AST in the blood serum, whereas it was increased in response to Cr2O3 and CrPic (300 and 500 µg/kg) administration (p ≤ 0.05). Stimulation of the activity of amylase and protease was observed in NP-treated animals, and a decrease was induced by CrCl3 (500 µg/kg) and CrPic (300 µg/kg). The reaction of the intestinal microbiota was characterized by the growth of enterobacteria, lactobacilli at day 14 except for the groups receiving CrPic 300 and NP 500 µg/kg; the number of bifidobacteria decreased in all experimental groups, except for CrCl3 and CrPic (500 µg/kg). Therefore, 300 and 500 µg/kg NP can be used as an antibacterial agent and as growth and hemopoiesis stimulant.Correspondence to:
Svyatoslav Lebedev, PhD, DSc
Federal Scientific Center “Biological Systems and Agrotechnologies RAS”
January 9 St., 29, Orenburg, 460000, Russia
Email: [email protected]
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Analysis of the hair toxic elements in various ethnic groups of the North-East of Russia
Elena A. Lugovaya and Anatoly L. Gorbachev
Price
42.00 $
Page No. 246
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (246-248)
Analysis of the hair toxic elements in various ethnic groups of the North-East of Russia
Elena A. Lugovaya1 and Anatoly L. Gorbachev2
1Scientific Research Center “Arktika” FEB RAS and 2North-Eastern State University, Magadan, Russia
The aim of the research was to estimate the hair levels of toxic elements in inhabitants of the North-Eastern region of Russia taking into account the different adaptive potential of the indigenous and newcoming population of the North. The hair lead, cadmium, mercury, as well as calcium and zinc content was studied using atomic emission and mass spectrometry in representatives of various ethnic groups aged 18 – 65 years old (Koryaks, n = 40; Chukchee, n = 35; Evens, n = 68; Caucasians, n = 89) living in Magadan Region. In the Evens, hair cadmium levels exceeded that of the Koryaks, Chukchee, and Caucasians, whereas hair lead values in the Chukchee were lower than in the other studied groups. There were no significant ethnic differences in mercury content. Therefore, Magadan Region can be counted among the territories with a relative favorable environmental situation with regard to the burden of toxic metals.Correspondence to:
Elena A. Lugovaya, PhD, Assistent Director
Scientific Research Center “Arktika” FEB RAS
Magadan, 680000, Russia
Email: [email protected]
Abstracts
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Organizers: Russian Society for Trace Elements in Medicine (RUSTEM), Moscow, Russia; Trace Element Institute for UNESCO, Lyon, France; Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia
Page No. 249
Abstract
Trace Elements and Electrolytes, Vol. 35 – No. 4/2018 (249-254)
V. Congress of the Russian Society for Trace Elements in Medicine (RUSTEM)
Organizers: Russian Society for Trace Elements in Medicine (RUSTEM), Moscow, Russia; Trace Element Institute for UNESCO, Lyon, France; Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia
