Abstract

Objective: To investigate the expression of monocyte chemoattractant protein-1 (MCP-1) in the brain tissue of patients with intractable epilepsy (IE) and to explore its role in the pathogenesis of IE. Material: Patients with IE were collected from the Department of Neurosurgery of several Chinese hospitals between 2002 and 2005. The average age of these 40 patients was 23.65 ± 10.14 (X ± SD) years (11 – 58 years), with 19 males and 21 females. In addition to the typical symptoms and distinct EEG features of epilepsy, all recruited patients met the requirements of intractable epilepsy. Methods: On the basis of positive results obtained from gene chip analysis, the expression of MCP-1 was first investigated in the brain tissue of IE patients (40 cases) using Western Blotting and immunohistochemistry and then compared with the controls. Results: Gene chip scanning demonstrated that expression of MCP-1 mRNA was upregulated in the brain tissue of patients with IE. Western Blotting and immunohistochemical experiments further confirmed that, as compared with that in the control group, expression of MCP-1 protein was significantly increased in the IE patients (p < 0.05). Conclusion: These results suggest that MCP-1 are involved in the pathogenesis of IE.

Author Details

Authors

• Y. Wu
• X. Wang
• X. Mo
• Z. Xi¹
• F. Xiao
• J. Li
• X. Zhu
• G. Luan
• Y. Wang
• Y. Li
• J. Zhang

Departments

• ¹ Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing,
• ² Neurology Department of the First Affiliated Hospital, Guangxi Medical University, Nanning,
• ³ Beijing Tiantan Hospital of the Capital University of Medical Sciences,
• ⁴ Beijing Xuanwu Hospital of the Capital University of Medical Sciences, Beijing, China

Address

Dr. X. Wang; Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
Email: [email protected]

Log in for Subscribers

Citation