Abstract

Aim: By observing the expression and distribution of platelet-derived growth factor receptor α-positive (PDGFRα⁺) cells in ureteropelvic junction obstruction (UPJO), to explore their role in the pathogenesis of children with congenital hydronephrosis.
Materials and methods: The control group involved specimens of the normal ureter (nephrectomy for tumor; n = 10), and the UPJO group contained specimens of ureteropelvic junction (UPJ) segment excised during pyeloplasty (n = 30). The specimens were investigated using immunofluorescence for the expression and distribution of PDGFRα⁺ cells in each group by light microscopy with computerized image analysis. Real-time PCR (RT-PCR) was used to study PDGFRα gene expression levels. In addition, small conductance calcium-activated potassium channel 3 (SK3) and closely associated cells consisting of smooth muscle cells (SMCs), interstitial cells of Cajal (ICCs), and nerve fibers were investigated.
Results: PDGFRα⁺ cells were in close proximity to SMCs, ICCs, and nerve fibers. PDGFRα⁺ cells expressed SK3 channels, which are found to regulate purinergic inhibitory neurotransmission in SMCs. Regarding the expression of PDGFRα⁺ cells no significant difference was seen between the two groups, while the expression of SK3 channels in PDGFRα⁺ cells was significantly decreased in the UPJO group versus the control group.
Conclusion: This study identified the expression of PDGFRα⁺ cells in the human UPJ. Our results demonstrate the expression of SK3 channels in PDGFRα⁺ cells was decreased in UPJO, and SK3 channels may be involved in the pathogenesis of UPJO by perturbing the UPJ peristalsis.

Author Details

Authors

• TianYi Wang
• Weiping Zhang
• Wenwen Han

Departments

• Department of Urology, Beijing Children’s Hospital,Capital Medical University, Beijing, China

Address


Weiping Zhang, MD, PhD
Department of Urology, Beijing Children’s Hospital
No. 56 Nanlishi St, Xicheng District, Beijing, 100045, China
Email: [email protected]

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